Vitamin B3 / B5 (LCMSMS)

Nicotinic acid and its amide nicotinamide are the common forms of the B-vitamin niacin (vitamin B3). Pharmaceutical and supplemental niacin are primarily used to treat hypercholesterolemia (high cholesterol) and pellagra (niacin deficiency). Insufficient niacin in the diet can cause nausea, skin and mouth lesions, anemia, headaches, and tiredness.

Pantothenic acid, also called pantothenate (vitamin B5), is a water-soluble vitamin. Pantothenic acid is an essential nutrient. Pantothenic acid is required to synthesize coenzyme-A (CoA), as well as to synthesize and metabolize proteins, carbohydrates, and fats. Pantothenic acid is the amide between pantoic acid and β-alanine. Small quantities of pantothenic acid are found in nearly every food, with high amounts in avocado, whole-grain cereals, legumes, eggs, meat, royal jelly, and yogurt. It is commonly found as its alcohol analog, the provitamin panthenol (pantothenol), and as calcium pantothenate. Pantothenic acid is an ingredient in some hair and skin care products.

Technical data

Sample Serum, Plasma
Sample volume 200 µl
Detector LCMSMS AB Sciex API 4000
Method gradient
Determinations 100

Ordering Information

IC3500 Testkit
IC3500ko Controls (2 level each 0.25 ml lyoph.)
IC3500rp HPLC column

Principle of the method

For the determination of Vitamin B3/B5 a precipitation step, which removes high molecular substances is performed first. After centrifugation the supernatant is injected into the LCMSMS system.

The HPLC separation is performed by a gradient method with mobile phase A and B at 20-25° C with a HPLC-column (IC3500rp). Chromatograms are detected by an MSMS-detector (AB-Sciex API4000). The separation takes 6 minutes for each run depending on the column used. Results are quantified by the delivered calibrator and calculated by the “internal standard-method” by integration of the peak heights or areas.