In a healthy body oxidative and reductive processes are in a balance. Free radicals (reactive oxygen species) are eliminated by antioxidants. In case of a lack of antioxidants free radicals react with cell structures. A reaction with unsaturated fatty acids lead to lipid peroxidation products. These circumstances are under discussion during the genesis of atheriosclerosis. Recent findings suggest that also in inflammation, sepsis, cancerogenesis and neurodegenerative diseases free radicals are involved.
An increased level of peroxides in blood indicates oxidative stress in the body. The measurement of peroxides can indicate an increased risk for heart attack and stroke. A subsequent change in nutrition and/or supplementation therapy might reduce the risk.
The peroxide assay is rapid and easy to perform and detects the total lipid hydroperoxides.
Sample EDTA-plasma, serum
Sample volume 20 µl
Calibration 1 point
Incubation time 15 min
IC5100ko Controls (2 level each 250 µl lyoph.)
Principle of the method
The determination of the peroxides is performed by the reaction of a peroxidase with peroxides in the sample followed by the conversion of TMB to a colored product.
After addition of a stop solution the samples are measured at 450 nm in a microtiter plate reader. The quantification is performed by the delivered calibrator.