Alpha-1-Antitrypsin is a 52 kD glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitor. Alpha-1-antitrypsin inhibits, beside others, the proteinases trypsin and the elastase of neutrophiles. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of alpha-1-antitrypsin is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes.
Indikationen
Technical data
Sample Stool, serum
Sample volume 100 mg, 100 µl
Calibration 5 point
Incubation time 1h, 1h, 15 min
Method ELISA
Determinations 96
Ordering Information
IC6200 Testkit
Principle of the method
The α-1-AT -ELISA test determines human alpha-1-antitrypsin according to the “sandwich”-principle. α-1-AT in sample, standard and controls binds to antibodies, which are coated to the microtiterplate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm (against teh reference wavelength 620 nm) in a microtiterplate reader. The α-1-AT concentration can be calculated from the standard curve.