In a healthy body oxidative and reductive processes are in a balance. Free radicals (reactive oxygen species) are eliminated by antioxidants. In case of a lack of antioxidants free radicals react with cell structures. A reaction with unsaturated fatty acids lead to lipid peroxidation products. These circumstances are under discussion during the genesis of atheriosclerosis. Recent findings suggest that also in inflammation, sepsis, cancerogenesis and neurodegenerative diseases free radicals are involved.
For detoxification the the body has a lot of antioxidants, e.g. vitamin C, vitamin E, glutathion, uric acid, albumin and the enzymes superoxid dismutase, catalase, glutathion reductase and glutathion transferase.
The test determines the total capacity of the plasma to detoxify peroxides, which are reaction products of free radicals. It gives a good overview about the antioxidative protection system.
A change in nutrition and/or a supplementation will increase the antioxidative capacity which will reduce the risk of heart attack and stroke.
Sample EDTA-plasma, serum
Sample volume 40 µl
Calibration 1 point
Incubation time 15 min
IC5200ko Controls (2 level each 250 µl lyoph.)
Principle of the method
The determination of the antioxidative capacity is performed by the reaction of antioxidants in the sample with a defined amount of added hydrogen peroxide (H2O2) The antioxidants in the sample eliminate a certain amount of the provided hydrogen peroxide. The residual H2O2 is determined photometrically by an enzymatic reaction which involves the conversion of TMB to a colored product.
After addition of a stop solution the samples are measured at 450 nm in a microtiter plate reader. The quantification is performed by the delivered calibrator.